CD16 antibody

• Catalog Number: orb154424
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD16 which expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts. It is a receptor for the Fc region of IgG. CD16 is one of only four eukaryotic receptors known to exist natively in both the transmembrane and glycosylphosphatidylinositol isoforms.
• Clonality: Monoclonal
• Clone Number: 3G8
• Tested applications: FA, FC, IHC-Fr, IP
• Reactivity: Human, Primate
• Isotype: Mouse IgG1 kappa
• Immunogen: Human neutrophils
• Concentration: 1 mg/ml
• Dilution range: Functional application: In vitro stimulation of NK cell proliferation, blocking of IgG binding and phagocytosis, inhibition of cytotoxic activity, in vivo NK cell depletion. Flow cytometry: Recommended dilution: 6 μg/ml. Immunohistochemistry (frozen sections): Acetone fixation.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD16
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Alternative names: Anti-FcgammaRIII antibody, anti-IGFR3 antibody, an
• Note: For research use only
• Application notes: Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications
• Expiration Date: 12 months from date of receipt.

Separation of human CD16 positive lymphocytes (red-filled) from CD16 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD16 (3G8) purified antibody (concentration in sample 2 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD16 (3G8) purified antibody (concentration in sample 2 μg/ml, GAM APC).