CD15 Antibody / SSEA-1

• Catalog Number: orb2643287
• Category: Antibodies
• Description: CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on 95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgM, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: PEG precipitation
• Immunogen: Human U937 cells were used as the immunogen for the CD15 antibody.
• UniProt ID: P22083
• Tested applications: FACS, IF, IHC-P
• Dilution range: Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: SPM119
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This CD15 antibody is available for research use only.
• Note: For research use only

Flow cytometry testing of human monocytes with CD15 antibody (clone SPM119); Green=isotype control, Red= CD15 antibody.

Flow cytometry testing of human U937 cells with CD15 antibody (clone SPM119); Red=isotype control, Blue= CD15 antibody.

Immunofluorescent staining of human U937 cells with CD15 antibody (clone SPM119, green) and Reddot nuclear stain (red).

IHC staining of FFPE Hodgkins lymphoma with CD15 antibody (clone SPM119). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.