CD15 Antibody / SSEA-1

• Catalog Number: orb385763
• Category: Antibodies
• Description: CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on > 95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Leu-M1 or MMA
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgM, kappa
• Immunogen: The histiocytic cell line U937 was used as the immunogen for this CD15 antibody.
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Precipitated followed by dialysis in 1X PBS
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD15 antibody is available for research use only.
• UniProt ID: P22083
• Storage: Store the CD15 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The optimal dilution of the CD15 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human Hodgkin's lymphoma using CD15 antibody

Flow cytometric analysis of human Monocytes using CD15 antibody