CD15 Antibody / SSEA-1

• Catalog Number: orb2642081
• Category: Antibodies
• Description: CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on > 95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: FUT4/1478R
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG, kappa
• Immunogen: Purified human neutrophils were used as the immunogen for the recombinant CD15 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein A affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant CD15 antibody is available for research use only.
• UniProt ID: P22083
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant CD15 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of human U937 cells with recombinant CD15 antibody (clone FUT4/1478R, green) and Reddot nuclear stain (red).

IHC testing of FFPE human Hodgkin's lymphoma and recombinant CD15 antibody (clone FUT4/1478R). Required HIER: steam sections in 10 mM Tris with 1 mM EDTA, pH9.0, for 10-20 min.

FACS analysis of human U937 cells using recombinant CD15 antibody (clone FUT4/1478R, blue) and isotype control (red).