CD15 Antibody / SSEA-1

• Catalog Number: orb2641725
• Category: Antibodies
• Description: CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on > 95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgM, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: PEG precipitation
• Immunogen: Purified human neutrophils were used as the immunogen for the CD15 antibody.
• UniProt ID: P22083
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
• Application notes: Optimal dilution of the CD15 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: CDLA15-1
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This CD15 antibody is available for research use only.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human Hodgkin's lymphoma and CD15 antibody (clone CDLA15-1).