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Catalog Number | orb1474496 |
---|---|
Category | Antibodies |
Description | Rabbit monoclonal antibody to CD100 |
Species/Host | Rabbit |
Clonality | Monoclonal |
Tested applications | IF, WB |
Reactivity | Human |
Immunogen | A synthetic peptide of human Semaphorin 4D |
Dilution range | WB (1/500 - 1/1000), IF/IC (1/50 - 1/100) |
Form/Appearance | Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin. |
Conjugation | Unconjugated |
Target | SEMA4D |
Entrez | 10507 |
UniProt ID | Q92854 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin. |
Alternative names | C9orf164; CD100; SEMAJ; Semaphorin-4D; A8; BB18; G Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of CD100 expression in Jurkat (A) whole cell lysates. (Predicted band size: 96 kD; Observed band size: 120 kD)
Immunofluorescent analysis of CD100 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
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