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CCR7 Rabbit Polyclonal Antibody (BF647)

SKU: orb1607347

Description

CCR7 Rabbit Polyclonal Antibody (BF647)

Images & Validation

Tested ApplicationsFC, ICC, IF
Dilution rangeFlow-Cyt=1μg/Test, IF=1:100-500, ICC/IF=1:100-500
ReactivityHuman, Mouse, Rat
Predicted ReactivityCanine

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human CCR7 (25-59/379aa)
TargetCCR7
PurificationAffinity purified by Protein A

Storage & Handling

StorageShipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

CCR7_HUMAN; BLR 2; BLR2; C C chemokine receptor type 7; C C CKR 7; CC chemokine receptor 7; CC chemokine receptor type 7; CC CKR 7; CCCKR7; CCR 7; CD 197; CD197; CD197 antigen; CDW197; Chemokine C C motif receptor 7; Chemokine C C receptor 7; Chemokine receptor 7-like protein; EBI 1; EBI1; Ebi1h; EBV Induced G Protein Coupled Receptor 1; Epstein Barr virus induced G protein coupled receptor; Epstein Barr virus induced gene 1; EVI 1; EVI1; Lymphocyte Specific G Protein Coupled Peptide Receptor; MGC108519; MIP 3 beta receptor; MIP3 beta Receptor.

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CCR7 Rabbit Polyclonal Antibody (BF647)

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-CCR7 antibody (orb1607347), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

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Protocol Information

FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ICC
Immunocytochemistry
View Protocol

CCR7 Rabbit Polyclonal Antibody (BF647) (orb1607347)

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100 μl
$ 540.00
DispatchUsually dispatched within 2-3 weeks
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