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Catalog Number | orb1272750 |
---|---|
Category | Antibodies |
Description | Ccl4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, NeA, WB |
Reactivity | Mouse |
Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMIP-1β. Murine MIP-1β specific antibody was purified by affinity chromatography employing immobilized mMIP-1β matrix. |
Concentration | batch dependent |
Dilution range | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Murine MIP-1β (100.0 ng/mL), a concentration of 8.0-10.0 μg/mL of this antibody is required.ELISA:Indirect:To detect mMIP-1b by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-1b.SandwichTo detect mMIP-1b by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine MIP-1b as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-1b. Western Blot:To detect mMIP-1b by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-1b is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | Ccl4 |
UniProt ID | P14097 |
NCBI | P14097 |
Storage | MIP-1B antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | Act-2, Mip1b, Scya4, MIP-1B, AT744.1, C-C motif ch Read more... |
Note | For research use only |
Application notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Murine MIP-1β (100.0 ng/mL), a concentration of 8.0-10.0 μg/mL of this antibody is required.ELISA:Indirect:To detect mMIP-1b by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-1b.SandwichTo detect mMIP-1b by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine MIP-1b as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-1b. Western Blot:To detect mMIP-1b by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-1b is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |
To detect Murine MIP-1-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine MIP-1-beta (orb1272749) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine MIP-1-beta.
To detect Murine MIP-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Murine MIP-1-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Murine MIP-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Murine MIP-1-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA, IF, IHC-P, WB | |
Bovine, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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