CCL4 Antibody

• Catalog Number: orb1272371
• Category: Antibodies
• Description: CCL4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, NeA, WB
• Reactivity: Human
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIP-1-beta.
• Concentration: batch dependent
• Dilution range: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-1-beta (50.00 ng/mL), a concentration of 1.75 - 3.0 μg/mL of this antibody is required. ELISA:To detect hMIP-1-beta by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-1-beta.Sandwich:To detect hMIP-1β by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MIP-1βas a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1β. Western Blot:To detect hMIP-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: CCL4
• UniProt ID: P13236
• NCBI: P13236
• Storage: MIP-1B antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: ACT2, G-26, HC21, LAG1, LAG-1, MIP1B, SCYA2, SCYA4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

To detect Human MIP-1-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human MIP-1-beta (orb1272370) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-1-beta.

To detect Human MIP-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-1-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Human MIP-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-1-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.