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Catalog Number | orb1247242 |
---|---|
Category | Antibodies |
Description | CAV3 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Bovine, Canine |
Reactivity | Human, Mouse, Porcine, Rat |
Immunogen | The immunogen for this antibody is: EHTDLEAQIVKDIH-C |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 23kDa band observed in Human, Mouse and Rat Heart lysates and 20kDa in Pig heart and Skeletal Muscle lysates (calculated MW of 17.3kDa according to NP_203123.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Skeletal Muscle shows peripheral staining of fibres. Recommended concentration, 3-5ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | CAV3 |
UniProt ID | P56539 |
NCBI | NP_203123.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | CAV3, caveolin 3, LGMD1C, LQT9, MGC126100, MGC1261 Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 23kDa band observed in Human, Mouse and Rat Heart lysates and 20kDa in Pig heart and Skeletal Muscle lysates (calculated MW of 17.3kDa according to NP_203123.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Skeletal Muscle shows peripheral staining of fibres. Recommended concentration, 3-5ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1247242 (0.3 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1247242 (3.8 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb1247242 (0.1 ug/ml) staining of Pig Skeletal Muscle (A) and Heart (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1247242 (0.3 ug/ml) staining of Mouse (A) and Rat (B) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Bovine, Human, Mouse, Porcine, Rat | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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