Caveolin-3/CAV3 Antibody

• Catalog Number: orb546303
• Category: Antibodies
• Description: Caveolin-3/CAV3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Caveolin-3/CAV3 recombinant protein (Position: M1-D55).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunofluorescence, 2μg/ml Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 22 kDa
• UniProt ID: P56539
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Caveolin-3; M-caveolin; CAV3
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of CAV3 using anti-CAV3 antibody.Lane 1:human placenta tissue;2:human U-87MG cell;3:human HeLa cell;4:rat stomach tissue;5:rat testis tissue;6:mouse stomach tissue;7:mouse testis tissue.

IF analysis of CAV3 using anti-CAV3 antibody. CAV3 was detected in a paraffin-embedded section of human cardiac muscle tissue.

IF analysis of CAV3 using anti-CAV3 antibody. CAV3 was detected in a paraffin-embedded section of mouse cardiac muscle tissue.

IF analysis of CAV3 using anti-CAV3 antibody. CAV3 was detected in a paraffin-embedded section of rat cardiac muscle tissue.

IHC analysis of CAV3 using anti-CAV3 antibody. CAV3 was detected in a paraffin-embedded section of human skeletal muscle tissue.