Carbonic Anhydrase II/CA2 Antibody

• Catalog Number: orb371723
• Category: Antibodies
• Description: Carbonic Anhydrase II/CA2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CA2 recombinant protein (Position: S2-K260). Human CA2 shares 81.1% and 80.7% amino acid (aa) sequence identity with mouse and rat CA2, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Western blot, 0.1-0.5μg/ml, Human, Mouse, RatImmunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 29246 MW
• UniProt ID: P00918
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Carbonic anhydrase 2;4.2.1.1;Carbonate dehydratase
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HT-29 cells using anti-CA2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of SW480 cells using anti-CA2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CA2 using anti-CA2 antibody.Lane 1:human 293T cell; 2:human THP-1 cell; 3:human HEL cell; 4:rat kidney tissue; 5:rat stomach tissue; 6:mouse kidney tissue; 7:mouse stomach tissue.

IHC analysis of CA2 using anti-CA2 antibody.CA2 was detected in paraffin-embedded section of human gastric cancer tissues.

IHC analysis of CA2 using anti-CA2 antibody.CA2 was detected in paraffin-embedded section of human liver cancer tissues.