CA2 Antibody (monoclonal, 10E11)

• Catalog Number: orb527049
• Category: Antibodies
• Description: CA2 Antibody (monoclonal, 10E11)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 10E11
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG2b
• Immunogen: E.coli-derived human CA2 recombinant protein (Position: S2-K260). Human CA2 shares 81.1% and 80.7% amino acid (aa) sequence identity with mouse and rat CA2, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 5μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 28 kDa
• UniProt ID: P00918
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Carbonic anhydrase 2; Carbonate dehydratase II; Ca
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of 293T cells using anti-CA2 antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis using anti-CA2 antibody.Lane 1:rat heart tissue;2:rat kidney tissue;3:rat spleen tissue;4:rat liver tissue;5:mouse heart tissue;6:mouse kidney tissue;7:mouse spleen tissue;8:mouse liver tissue;9:human placenta tissue;10:human 293T cell.

IF analysis of CA2 using anti-CA2 antibody. CA2 was detected in immunocytochemical section of HEPG2 cells.

IHC analysis of CA2 using anti-CA2 antibody. CA2 was detected in paraffin-embedded section of human gastric cancer tissue.

IHC analysis of CA2 using anti-CA2 antibody. CA2 was detected in paraffin-embedded section of human colon cancer tissue.

IHC analysis of CA2 using anti-CA2 antibody. CA2 was detected in paraffin-embedded section of mouse intestine tissue.

IHC analysis of CA2 using anti-CA2 antibody. CA2 was detected in paraffin-embedded section of rat intestine tissue.