C5b-9 Rabbit Polyclonal Antibody (APC)

• Catalog Number: orb994688
• Category: Antibodies
• Description: C5b-9 Rabbit Polyclonal Antibody (APC)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF
• Predicted Reactivity: Mouse
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: Purified Human SC5b-9 Complex
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IF=1:100-500
• Conjugation: APC
• Target: C5
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: C5; C6; C7; C8; C9; Complement component 5; Comple
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-C5b-9 antibody (orb994688), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.