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Catalog Number | orb102206 |
---|---|
Category | Antibodies |
Description | C5b-9 Rabbit Polyclonal Antibody (FITC) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF |
Predicted Reactivity | Mouse |
Reactivity | Human, Rat |
Isotype | IgG |
Immunogen | Purified Human SC5b-9 Complex |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | IF=1:100-500 |
Conjugation | FITC |
Target | C5 |
Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | C5; C6; C7; C8; C9; Complement component 5; Comple Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Blank control: Hela. Primary Antibody (green line): Rabbit Anti-C5b-9 antibody (orb102206), Dilution: 2 ug/Test, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.