C22ORF28 antibody

• Catalog Number: orb22473
• Category: Antibodies
• Description: Goat Polyclonal to C22ORF28
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 5-10μg/ml
• Conjugation: Unconjugated
• MW: 55.2
• Target: HSPC117 (aa201-215)
• Entrez: 51493
• Protein Sequence: QADPNKVSARAKKR
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti -novel protein HSPC117 antibody, anti-hypothe
• Note: For research use only
• Application notes: WB: Approx 55kDa band observed in lysates of cell lines HeLa, Jurkat and K562 (calculated MW of 55.2kDa according to NP_055121.1). Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb22473 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

orb22473 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

orb22473 (0.03ug/ml) staining of Human Cerebellum (A) and Ovary (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

orb22473 (0.01ug/ml) staining of Jurkat (A), HeLa (B), HepG2 (C) and A431 (D) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

orb22473 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western blot analysis of HeLa lysate using C22ORF28 antibody.

Immunohistochemical staining of Human Testis using C22ORF28 antibody.