C-Kit/CD117 Rabbit Polyclonal Antibody

SKU: orb500838

Description

C-Kit/CD117 Rabbit Polyclonal Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Reactivity: Human, Rat
• Predicted Reactivity: Canine, Equine, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human c-Kit/CD117 (301-400/976aa)
• Target: KIT
• Molecular Weight: 105 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

C-Kit; CD117; MASTC; PBT; SCFR; Bs; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; SCO1; SCO5; SOW3; Ssm; Tr-kit; W; KIT_HUMAN; KIT; Piebald trait protein (PBT); Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; 2.7.10.1; KIT_MOUSE; Sl;

Blank control (blue line): K562 (fixed with 70% ethanol (overnight at 4°C) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-c-Kit antibody (orb500838), dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (c-Kit/CD117) Polyclonal Antibody, Unconjugated (orb500838) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

KIT

UniProt

P10721