C-Kit/CD117 Rabbit Polyclonal Antibody (APC)

• Catalog Number: orb1000669
• Category: Antibodies
• Description: C-Kit/CD117 Rabbit Polyclonal Antibody (APC)
• Target: KIT
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: APC
• Reactivity: Human, Rat
• Predicted Reactivity: Canine, Equine, Mouse
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human c-Kit/CD117 (301-400/976aa)
• Tested applications: FC, IF
• Dilution range: Flow-Cyt=1ug/Test, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: C Kit; c-Kit; CD 117; CD117; CD117 antigen; KIT; K
• Research Area: Cancer Research, Cell Biology, Cell Markers, Devel
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: K562. Primary Antibody (green line): Rabbit Anti-c-Kit antibody (orb1000669), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.