BrdU antibody

• Catalog Number: orb1474636
• Category: Antibodies
• Description: Rabbit polyclonal antibody to BrdU
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH
• Reactivity: All
• Immunogen: BrdU
• Dilution range: IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Bromodeoxyuridine
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of BrdU staining in U2OS treated with BrdU. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.