BrdU Antibody [BRD469]

• Catalog Number: orb1252671
• Category: Antibodies
• Description: BrdU Antibody [BRD469]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BRD469
• Tested applications: FC, IF, IHC
• Reactivity: All
• Isotype: IgG1
• Immunogen: Bromodeoxyuridine conjugated to KLH was used as the immunogen.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine antibody to be titered up or down for optimal performance.1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine antibody to be titered up or down for optimal performance.1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC.
• Expiration Date: 12 months from date of receipt.

IHC staining of mouse intestine tissue (10X) with BrdU antibody (BRD469).

IHC staining of mouse intestine tissue (20X) with BrdU antibody (BRD469).

Immunofluorescence testing of rat kidney with Alexa Fluor 488 conjugated BrdU antibody.