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Catalog Number | orb154393 |
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Category | Antibodies |
Description | Mouse monoclonal antibody to BrdU |
Clonality | Monoclonal |
Clone Number | Bu20a |
Tested applications | FC, IHC-Fr, IHC-P |
Isotype | Mouse IgG1 |
Immunogen | Bromodeoxyuridine conjugated to BSA |
Concentration | 1 mg/ml |
Dilution range | Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining. |
Purity | Purified by protein-A affinity chromatography. |
Conjugation | Unconjugated |
Target | BrdU |
Storage | Store at 2-8°C. Do not freeze. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | anti Bromodeoxyuridine antibody, anti BUdr antibod Read more... |
Note | For research use only |
Application notes | Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining. |
Expiration Date | 12 months from date of receipt. |
Flow cytometry intracellular staining pattern of BrdU incorporated K562 cells stained using anti-BrdU (Bu20a) purified antibody (concentration in sample 4 μg/ml, GAM FITC).
Flow cytometry multicolor intracellular staining pattern of BrdU incorporated K562 cellular suspension using anti-BrdU (Bu20a) purified antibody (concentration in sample 4 μg/ml, GAM FITC) and 7-AAD.
Separation of human BrdU positive cells (red-filled) from cellular debris (black-dashed) in flow cytometry analysis (intracellular staining) of BrdU incorporated K562 cells stained using anti-BrdU (Bu20a) purified antibody (concentration in sample 4 μg/ml, GAM FITC).
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