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Catalog Number | orb344394 |
---|---|
Category | Antibodies |
Description | Biotin antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 4C7.D5 |
Tested applications | ELISA, FC, IF, IHC, WB |
Isotype | IgG2a |
Immunogen | Anti-Biotin Antibody was produced by repeated immunizations with Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH). |
Concentration | 1.0 mg/ml |
Dilution range | ELISA: 1:20,000, FC: 1-2 µg/mL, IHC: 1:1,000 - 1:5,000, IF: 2 ug/mL, WB: 1:2,000 - 1:10,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-Biotin Antibody was prepared from mouse ascites by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin. |
Conjugation | Unconjugated |
Storage | Store Anti-Biotin at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | mouse anti-Biotin Monoclonal Antibody, Biotin Anti Read more... |
Note | For research use only |
Application notes | Anti-Biotin antibody has been tested in ELISA, western blot and immunofluorescence and is suitable for lateral flow, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. |
Expiration Date | 12 months from date of receipt. |
ELISA, IHC | |
Rabbit | |
Goat | |
Polyclonal | |
Biotin |
ELISA, FC, IF, IHC, IP, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Biotin |
DOT, ELISA, FC, IHC, IP, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Biotin |
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