Biotin Antibody

• Catalog Number: orb1786031
• Category: Antibodies
• Description: Biotin Antibody
• Clonality: Monoclonal
• Clone Number: 1D4-C5
• Tested applications: CyTOF, ELISA, FC, IP
• Isotype: Mouse IgG2a kappa
• Immunogen: biotinylated limulus hemocyanin
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: Biotin
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: vitamin H, vitamin B7
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA analysis of a biotinylated antigen using biotin-specific antibody and streptavidin. Microtitration wells were coated with biotinylated human IgG (0.01 μg/ml) and blocked. Then either anti-biotin mouse monoclonal 1D4-C5 (A, C) or streptavidin-HRP (B) was added in six different doses. Finally, goat anti-mouse-HRP conjugate (A) or streptavidin-HRP (C) was added. HRP signal increased in dose dependent manner, but with different dynamics, when biotinylated antibody was detected by anti-biotin mAb/goat anti mouse-HRP (A) or by streptavidin-HRP (B), and it was falling to zero in dose dependent manner when streptavidin-HRP was competed by anti-biotin mAb 1D4-C5 (C).