BCL2 Antibody

• Catalog Number: orb1252825
• Category: Antibodies
• Description: BCL2 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 100/D5
• Tested applications: ELISA, FC, IF, IHC, IP, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Amino acids GAAPAPGIFSSQPG (41-54) of the human protein were used as the immunogen for this Bcl-2 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: ELISA: order BSA-free format for coatingFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIP: 1-2ug/0.5mg lysateIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bcl-2 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: BCL2
• UniProt ID: P10415
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Bcl-2 Antibody: Bcl-2, PPP1R50, Apoptosis regulato
• Note: For research use only
• Application notes: ELISA: order BSA-free format for coatingFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIP: 1-2ug/0.5mg lysateIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bcl-2 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of formalin-paraffin non-Hodgkin's lymphoma stained with Bcl-2 antibody (100/D5). Note nuclear membrane & cytoplasmic staining.

Western blot testing of human samples using 100/D5 antibody.

FACS staining (intracellular) of Jurkat cells using 100/D5 antibody (red) and isotype control (green).