BAK/BAK1 Antibody

• Catalog Number: orb308779
• Category: Antibodies
• Description: BAK/BAK1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human BAK recombinant protein (Position: A22-S211). Human BAK shares 78.3 % amino acid (aa) sequence identity with mouse BAK.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 23409 MW
• UniProt ID: Q16611
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Bcl-2 homologous antagonist/killer;Apoptosis regul
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of THP-1 cells using anti-BAK antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of BAK using anti-BAK antibody.Lane 1:rat skeletal muscle tissue;2:rat lung tissue;3:mouse heart tissue;4:mouse skeletal muscle tissue;5:mouse lung tissue.

WB analysis of BAK using anti-BAK antibody.Lane 1:human PC-3 cell;2:human HEK293 cell;3:human Jurkat cell;4:human Caco-2 cell;5:human U2OS cell;6:human HepG2 cell;7:human HeLa cell;8:human A549 cell.

IF analysis of BAK using anti-BAK antibody. BAK was detected in immunocytochemical section of MCF-7 cells.

IHC analysis of BAK using anti-BAK antibody.BAK was detected in paraffin-embedded section of Human Intestinal Cancer Tissue.

IHC analysis of BAK using anti-BAK antibody. BAK was detected in paraffin-embedded section of Rat Skeletal Muscle Tissue.

IHC analysis of BAK using anti-BAK antibody.BAK was detected in paraffin-embedded section of Mouse Skeletal Muscle Tissue.

IHC analysis of BAK using anti-BAK antibody.BAK was detected in immunocytochemical section of A549 cell.

IHC analysis of BAK using anti-BAK antibody.BAK was detected in frozen section of rat cardiac muscle tissue.

IHC analysis of BAK using anti-BAK antibody.BAK was detected in immunocytochemical section of SMMC-7721 cell.

IHC analysis of BAK using anti-BAK antibody.BAK was detected in frozen section of human placenta tissue.

IHC analysis of BAK using anti-BAK antibody.BAK was detected in frozen section of mouse cardiac muscle tissue.