Bcl-2 Antibody

• Catalog Number: orb1524399
• Category: Antibodies
• Description: Bcl-2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, IP
• Reactivity: Human
• Immunogen: Between 50 and 100
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. orb1524399 has not performed satisfactorily when used for WB of Bcl-2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Bcl-2.; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Bcl-2
• UniProt ID: P10415
• NCBI: NP_000624.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: Bcl-2; B-cell CLL/lymphoma 2; apoptosis regulator
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Bcl-2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Bcl-2 antibody orb1524399 used for IP at 6 µg/mg lysate.

Detection of human Bcl-2 by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Bcl-2 (Cat. No. orb1524399) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human Bcl-2 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Bcl-2 (Cat. No. orb1524399) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB