BAX Antibody

• Catalog Number: orb1252035
• Category: Antibodies
• Description: BAX Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ARBX-1
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Recombinant full-length human protein was used as the immunogen for the Bax antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Immunofluorescence: 1-2 ug/mlWestern blot: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Bax antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: BAX
• UniProt ID: Q07812
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Bax Antibody: BCL2L4, BCL2L4, Apoptosis regulator
• Note: For research use only
• Application notes: Immunofluorescence: 1-2 ug/mlWestern blot: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Bax antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

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