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Catalog Number | orb1274527 |
---|---|
Category | Antibodies |
Description | BAX Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BAX (NP_620116.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:100 - 1:200IP: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 20kDa |
Target | BAX |
UniProt ID | Q07812 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | Bax Antibody: BCL2L4, BCL2L4, Apoptosis regulator Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:100 - 1:200IP: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry of paraffin-embedded mouse brain using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using BAX antibody (orb1274527) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using BAX antibody (orb1274527) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using BAX antibody (orb1274527) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using BAX antibody (orb1274527) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using BAX antibody (orb1274527) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using BAX antibody (orb1274527) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using BAX antibody (orb1274527) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using BAX antibody (orb1274527) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts from normal (control) and BAX knockout (KO) 293T cells, using BAX antibody (orb1274527) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
FC, ICC, IHC-P, WB | |
Bovine, Canine, Porcine, Sheep | |
Human, Mouse, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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