BACE1 Protein, Human, Recombinant (His)

Catalog Number: orb1958830

Catalog Number	orb1958830
Category	Proteins
Description	BACE1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 49.9 kDa and the accession number is A0A024R3D7.
Tag	C-His
Purity	98.00%
MW	49.9 kDa (predicted); 60-65 kDa (reducing condition, due to glycosylation)
UniProt ID	P56817
Expression System	HEK293 Cells
Biological Origin	Human
Biological Activity	BACE1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 49.9 kDa and the accession number is A0A024R3D7.
Expression Region	A DNA sequence encoding the extracellular domain (amino acid residue Met 1-Thr 457) of human BACE1 (NP_036236.1) precursor was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Thr 22 and Glu 46 (rare)
Storage	-20°C
Note	For research use only
Application notes	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Expiration Date	6 months from date of receipt.

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Purity:	The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 49.3 kDa after removal of the signal peptide. The apparent molecular mass of BACE1-His is approximately 55-100 kDa due to glycosylation.
Source:	Mammalian

Sizes Available: 100 μg, 10 μg, 50 μg

Purity:	SDS-PAGE: > 95%
MW:	98.9 kDa (predicted). Due to glycosylation, the protein migrates to 145-150 kDa based on Tris-Bis PAGE result.

Purity:	98.00%
MW:	100.7 kDa (predicted). Due to glycosylation, the protein migrates to 170-200 kDa based on Tris-Bis PAGE result.