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Catalog Number | orb1252471 |
---|---|
Category | Antibodies |
Description | B2M Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | B2M/961 |
Tested applications | FC, IF, IHC-P |
Reactivity | Human, Primate |
Isotype | IgG2b, kappa |
Immunogen | Full length recombinant human protein was used as the immunogen for the Beta-2 Microglobulin antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Beta-2 Microglobulin antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | B2M |
UniProt ID | P61769 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | B2M Antibody: Beta-2-microglobulin Read more... |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Beta-2 Microglobulin antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Beta-2-Microglobulin antibody (B2M/961).
Formalin-fixed, paraffin-embedded human melanoma stained with Beta-2-Microglobulin antibody.
Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Beta-2-Microglobulin antibody.
Formalin-fixed, paraffin-embedded human renal carcinoma stained with Beta-2-Microglobulin antibody.
Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin antibody.
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Beta-2-Microglobulin antibody.
Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin antibody.
ELISA, FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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