ATG5 antibody

• Catalog Number: orb411603
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ATG5
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human ATG5
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ATG5
• Entrez: 11793, 9474
• UniProt ID: Q9H1Y0, Q99J83, Q3MQ06
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-APG5L antibody, anti-ASP antibody, anti-Autop
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ATG5 expression in Hela (A), NIH3T3 (B), PC12 (C), rat bain (D) whole cell lysates. (Predicted band size: 22; 32 kD; Observed band size: 55 kD)

Immunofluorescent analysis of ATG5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).