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Catalog Number | orb33366 |
---|---|
Category | Antibodies |
Description | Purified rabbit polyclonal antibody. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB7466 |
Tested applications | IF, IHC-P, WB |
Predicted Reactivity | Porcine, Rat, Zebrafish |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Immunogen | Synthetic Peptide |
Dilution range | WB: 1:1000, IF/ICC: 1:200, IHC-P: 1:50-100 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 32447 |
Target | ATG5 |
UniProt ID | Q9H1Y0 |
NCBI | NP_004840.1 |
Alternative names | Anti-APG5 antibody, anti-APG5-LIKE antibody, anti- Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Kessel, David et al. Effects of Combined Lysosomal and Mitochondrial Photodamage in a Non-small-Cell Lung Cancer Cell Line: The Role of Paraptosis Photochem Photobiol, 93, 1502-1508 (2017)
Ozturk, Melek et al. The Detection Techniques for Autophagy-Associated Cell Death-Related Genes and Proteins: Gene Expression Assay and Immunohistochemistry Methods Mol Biol, 1854, 119-130 (2019)
Western blot analysis of 293 cell lysateusing ATG5 antibody (primary antibody at 1:1000)
Western blot analysis of 293 cell lysateusing ATG5 antibody (primary antibody dilution at: 1:1000)
Immunofluorescense analysis of U251 cells using ATG5 antibody (primary antibody dilution at: 1:200)
Immunohistochemical staining of paraffin embedded human cancer tissue using ATG5 antibody (primary antibody dilution at: 1:50-100)
Western blot analysis of Hela cell line lysates (35ug/lane)using ATG5 antibody (dilution of primary antibody at 1:1000)
Western blot analysis of mouse brain tissue lysatesusing ATG5 antibody (antibody dilution at 1:1000)
IHC-P, WB | |
Animal, Bovine, Canine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish | |
Canine, Equine, Guinea pig, Human, Mouse, Rat, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Bovine, Canine, Equine, Gallus, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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