ATG16L1 Antibody

SKU: orb19825

Description

Goat polyclonal antibody to ATG16L1

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.01-0.1 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 48kDa band in Human Kidney, Liver and Spleen lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 68.3kDa according to NP_110430.5 and of 66.3kDa according to NP_060444.3. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

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Key Properties

• Clonality: Polyclonal
• Target: ATG16L1
• Protein Sequence: KVEKVLSKQHSSSIN
• Molecular Weight: 68.3, 66.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ATG16 antibody, anti autophagy related 16-like 1 (S. cerevisiae) antibody, anti hCG_1817841 antibody, anti APG16L antibody, anti ATG16L antibody, anti FLJ00045 antibody, anti FLJ10035 antibody, anti FLJ10828 antibody, anti FLJ22677 antibody, anti WDR30 antibody, anti APG16 autophagy 16-like antibody, anti APG16L beta antibody, anti ATG16 autophagy related 16-like protein 1 antibody, anti WD repeat domain 30 antibody

Quick Database Links

Gene Symbol

ATG16L1

NCBI Gene ID

55054