ATF2 antibody

• Catalog Number: orb18311
• Category: Antibodies
• Description: Goat polyclonal antibody to ATF2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine, Canine, Human, Rat
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml
• Conjugation: Unconjugated
• MW: 54.5
• Target: ATF2
• Entrez: 1386
• Protein Sequence: IVMAPSSQSQPSGS
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti ATF2 antibody, anti activating transcription
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Experiments gave bands at approx 55kDa and 30kDa in lysates of Human Placenta and of cell line HeLa after 0.5 μg/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Bailey et al , J Clin Endocrinol Metab. 2002 Apr;87(4):1717-28.; PMID: 11932306). This protein has a calculated MW of 54.5kDa according to NP_001871.2. Recommended concentration: 0.5-1.5 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Human Placenta lysate using ATF2 antibody

Western blot analysis of HepG2 (Lane 1), K562 (Lane 2) and HeLa (Lane 3) nuclear lysates using ATF2 antibody