ATF2 Antibody

• Catalog Number: orb215886
• Category: Antibodies
• Description: ATF2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human ATF2 recombinant protein (Position: E93-E450). Human ATF2 shares 99% amino acid (aa) sequence identity with both mouse and rat ATF2.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 54537 MW
• UniProt ID: P15336
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Cyclic AMP-dependent transcription factor ATF-2;cA
• Note: For research use only
• Application notes: WB: The detection limit for ATF2 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of K562 cells using anti-ATF2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of HepG2 cells using anti-ATF2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of ATF2 using anti-ATF2 antibody.Lane 1:human HepG2 cell; 2:human K562 cell; 3:human SH-SY5Y cell; 4:human U87 cell; 5:human A549 cell; 6:human MOLT4 cell; 7:human HEL cell.

WB analysis of ATF2 using anti-ATF2 antibody.Lane 1:rat brain tissue; 2:rat thymus tissue; 3:mouse brain tissue; 4:mouse thymus tissue.

IHC analysis of ATF2 using anti-ATF2 antibody. ATF2 was detected in paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of ATF2 using anti-ATF2 antibody. ATF2 was detected in paraffin-embedded section of mouse brain tissue.

IHC analysis of ATF2 using anti-ATF2 antibody. ATF2 was detected in paraffin-embedded section of rat brain tissue.

IHC analysis of ATF2 using anti-ATF2 antibody.ATF2 was detected in frozen section of rat brain tissue.

IHC analysis of ATF2 using anti-ATF2 antibody.ATF2 was detected in frozen section of mouse brain tissue.