ARNT Antibody

• Catalog Number: orb1526033
• Category: Antibodies
• Description: ARNT Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: between 575 and 625
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ARNT
• UniProt ID: P27540
• NCBI: NP_001659.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: HIF-1beta; class E basic helix-loop-helix protein
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human ARNT by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ARNT antibody orb1526033 used for IP at 3 µg/mg lysate.

Detection of human ARNT by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ARNT (Cat. No. orb1526033) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB