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Catalog Number | orb639760 |
---|---|
Category | Antibodies |
Description | The ADP-ribosylation factor (ARF) family comprises a group of structurally and functionally conserved proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. The ARF family is divided functionally into the ARF and the ARF-like proteins. ARF's share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. ARF is involved in intracellular protein traffic to and within the Golgi complex. ARF has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | ARF1/2117 |
Tested applications | IHC-P |
Reactivity | Human, Mouse, Rat |
Isotype | Mouse IgG2a, kappa |
Immunogen | A recombinant full-length human ARF1 protein was used as the immunogen for this ARF1 antibody. |
Antibody Type | Primary Antibody |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This ARF1 antibody is available for research use only. |
UniProt ID | P84077 |
Storage | Store the ARF1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human brain with ARF1 antibody (clone ARF1/2117). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver with ARF1 antibody (clone ARF1/2117). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast with ARF1 antibody (clone ARF1/2117). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney with ARF1 antibody (clone ARF1/2117). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain with ARF1 antibody (clone ARF1/2117). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free ARF1 antibody (clone ARF1/2117) as confirmation of integrity and purity.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, IP, WB | |
Bovine, Canine, Equine, Guinea pig, Rabbit, Sheep, Yeast, Zebrafish | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |