Anti-TROP2 Reference Antibody (sacituzumab)

• Catalog Number: orb1818271
• Category: Antibodies
• Description: Recombinant Antibody
• Clonality: Monoclonal
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: IgG1
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Form/Appearance: Purified monoclonal antibody supplied in PBS, pH 6.0, without preservative.This antibody is purified through a protein A column.
• Conjugation: Unconjugated
• MW: 145.52
• UniProt ID: P09758
• Protein Sequence: TROP2
• Expression System: CHO Cell
• Endotoxins: < 0.001EU/μg,determined by LAL method.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Tumor-associated calcium signal transducer 2, Cell
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

The purity of Anti-TROP2 Reference Antibody (sacituzumab) is more than 96.23%, determined by SEC-HPLC.

Immobilized human TROP2 His at 2 µg/mL can bind Anti-TROP2 Reference Antibody (sacituzumab), EC50=0.004316 µg/mL

Anti-TROP2 Reference Antibody (sacituzumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%

The endocytosis ratio sacituzumab by human TROP2 HEK293 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 70% at antibody concentration of 0.4 nM.

Human TROP2 HEK293 cells were stained with Anti-TROP2 Reference Antibody (sacituzumab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC276=0.4071 µg/mL