Anti-TROP2 Reference Antibody (sacituzumab)

• Catalog Number: orb1806096
• Category: Antibodies
• Description: Anti-TROP2 Reference Antibody is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.
• Clonality: Monoclonal
• Tested applications: ELISA, FA, FACS, Kinetics
• Reactivity: Human
• Isotype: IgG
• Purity: > 95%
• Conjugation: Unconjugated
• MW: 145.5 kDa
• Target: TROP2
• Endotoxins: < 1 EU/mg
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100 mM Pro-Ac 20 mM Arg pH 5.0
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-TROP2 Reference Antibody (sacituzumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-TROP2 Reference Antibody (sacituzumab)is more than 96.23% ;determined by SEC-HPLC.

Immobilized human TROP2 His at 2 μg/mL can bind Anti-TROP2 Reference Antibody (sacituzumab);EC50=0.004316μg/mL.

Human TROP2 HEK293 cells were stained with Anti-TROP2 Reference Antibody (sacituzumab) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC276=0.4071 μg/mL.

The endocytosis ratio sacituzumab by human TROP2 HEK293 increased with the increase of antibody concentration; and the Internalization Rate (%) reached 70% at antibody concentration of 0.4 nM.