Anti-TIGIT Reference Antibody (tiragolumab)

• Catalog Number: orb1818428
• Category: Antibodies
• Description: Recombinant Antibody
• Clonality: Monoclonal
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human, Monkey, Rabbit
• Form/Appearance: Purified monoclonal antibody supplied in PBS, pH 6.0, without preservative.This antibody is purified through a protein A column.
• Concentration: 1mg/ml
• Protein Sequence: TIGIT
• UniProt ID: Q495A1
• MW: 148.66
• Tested applications: ELISA, FC
• Antibody Type: Primary Antibody
• Expression System: CHO Cell
• Endotoxins: < 0.001EU/μg,determined by LAL method.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: T-cell immunoreceptor with Ig and ITIM domains, V-
• Note: For research use only

The purity of Anti-TIGIT Reference Antibody (tiragolumab) is more than 95.79%, determined by SEC-HPLC.

Immobilized human TIGIT His at 2 µg/mL can bind Anti-TIGIT Reference Antibody (tiragolumab), EC50=0.01643 µg/mL

Anti-TIGIT Reference Antibody (tiragolumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%

Anti-TIGIT Reference Antibody (tiragolumab) -induced TIGIT Luciferase activity was evaluated using hu-TIGIT-CD226-NFAT-Jurkat, Reporter Cell. The EC50 was approximately 4.978nM.

Human TIGIT HEK293 cells were stained with Anti-TIGIT Reference Antibody (tiragolumab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC111=0.2053 µg/mL