Anti-SMAD1 Antibody

• Catalog Number: orb736926
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SMAD1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: SMAD1; SMAD5; SMAD9
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: WB: 1/500 - 1/1000
• Conjugation: Unconjugated
• UniProt ID: Q15797
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-SMAD1 antibody, anti-BSP1 antibody, anti-MADH
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SMAD1 expression in A375 (A), H460 (B) whole cell lysates.

Immunohistochemical analysis of SMAD1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.