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Anti-S100-A4 Antibody

Catalog Number: orb382023

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb382023
CategoryAntibodies
DescriptionRabbit polyclonal antibody to S100A4.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenRecombinant full length protein of human S100-A4
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-2000, IHC-P: 1:50-200
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetS100A4
Entrez24615, 20198, 6275
UniProt IDP26447, P05942, P07091
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesCAPL; MTS1; Protein S100-A4; Calvasculin; Metastas
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-S100-A4 Antibody

Western blot analysis of S100-A4 expression in Hela (A), NIH3T3 (B), HepG2 (C) whole cell lysates. (Predicted band size: 11 kD; Observed band size: 12 kD)

Anti-S100-A4 Antibody

Immunohistochemical analysis of S100-A4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-S100-A4 Antibody

Immunofluorescent analysis of S100-A4 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

  • Anti-S100-A4 Antibody [orb1474497]

    IF,  IH,  IP,  WB

    Human

    Rabbit

    Monoclonal

    Unconjugated

    200 μl, 100 μl, 30 μl
  • Anti-S100-A4 Antibody [orb705476]

    IH

    Human

    Mouse

    Monoclonal

    Unconjugated

    200 μl, 30 μl, 100 μl
  • Anti-S100-A4 Antibody [orb705502]

    IH

    Human

    Mouse

    Monoclonal

    Unconjugated

    30 μl, 100 μl, 200 μl
  • Anti-S100-A4 Antibody [orb1474272]

    IP,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    200 μl, 100 μl, 30 μl