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S100A4 Rabbit Polyclonal Antibody

Catalog Number: orb6910

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb6910
CategoryAntibodies
DescriptionS100A4 Rabbit Polyclonal Antibody
TargetS100A4
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Predicted ReactivityBovine, Canine, Equine, Guinea pig, Porcine, Rabbit
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthetic peptide derived from human S100A4 (15-101/101aa)
UniProt IDP26447
RRIDAB_10933874
MW12 kDa
Tested applicationsFC, IF, IHC-Fr, IHC-P
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesFSP1; FSP-1; FSP 1; 18A2; 42A; calcium Placental p
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NoteFor research use only
S100A4 Rabbit Polyclonal Antibody

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-S100A4 antibody (orb6910), dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

S100A4 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (S100A4) Polyclonal Antibody, Unconjugated (orb6910) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

S100A4 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (S100A4) Polyclonal Antibody, Unconjugated (orb6910) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

S100A4 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (S100A4) Polyclonal Antibody, Unconjugated (orb6910) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

S100A4 Rabbit Polyclonal Antibody

Sample: Hela Cell lysate at 30 ug, Primary: Anti-S100A4 (orb6910) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-Rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 12 kD, Observed band size: 15 kD.

S100A4 Rabbit Polyclonal Antibody

Sample: Raji Cell lysate at 30 ug, Primary: Anti-S100A4 (orb6910) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:5000 dilution, Predicted band size: 12 kD, Observed band size: 12 kD.

S100A4 Rabbit Polyclonal Antibody

Tissue/Cell: human lung cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-S100A4 Polyclonal Antibody, Unconjugated (orb6910) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    ELISA,  IF,  IHC,  WB

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    ELISA,  IHC,  WB

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