Anti-NDR1 Antibody

• Catalog Number: orb382063
• Category: Antibodies
• Description: Rabbit polyclonal antibody to STK38.
• Target: STK38
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant full length protein of human NDR1
• UniProt ID: Q15208, Q91VJ4
• Tested applications: IF, WB
• Dilution range: WB: 1:500-2000
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NDR1; Serine/threonine-protein kinase 38; NDR1 pro
• Note: For research use only
• Entrez: 11329, 106504

Western blot analysis of NDR1 expression in A549 (A), SW480 (B), 293T (C), MCF7 (D), mouse liver (E) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 62 kD)

Immunofluorescent analysis of NDR1 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).