Anti-IL-6 Antibody

• Catalog Number: orb1422415
• Category: Antibodies
• Description: Rabbit polyclonal antibody to IL-6.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• UniProt ID: P05231
• Tested applications: IHC, WB
• Dilution range: WB: WB (1/500 - 1/1000), IH (1/50 - 1/200)
• Clone Number: IL6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: IFNB2; Interleukin-6; IL-6; B-cell stimulatory fac
• Note: For research use only

Immunohistochemical analysis of IL-6 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of IL-6 expression in Hela (A), U87MG (B), HuT78 (C), A549 (D), MCF7 (E) whole cell lysates.