Anti-Histamine H2 Receptor Antibody

• Catalog Number: orb1423509
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histamine H2 Receptor.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: HRH2
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: WB: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IF: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
• Conjugation: Unconjugated
• UniProt ID: P25021
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Histamine H2 receptor; H2R; HH2R; Gastric receptor
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of Histamine H2 Receptor staining in COLO205 Cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of Histamine H2 Receptor expression in mouse kidney (A), rat liver (B) whole cell lysates.