Helios Antibody

• Catalog Number: orb222726
• Category: Antibodies
• Description: Hamster monoclonal antibody to Helios
• Target: Helios
• Clonality: Monoclonal
• Isotype: Armenian Hamster IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: Peptide coresponding to the amino acids 51-107 of Helios
• UniProt ID: Q9UKS7
• Tested applications: FC
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml.,Recommended protocol: 1) Perform staining of cell surface markers (CD25, CD4 etc.) for 20 min. at room temperature in the dark; 100 μl of peripheral blood. 2) Add 3 ml of PBS with 1% BSA, centrifugate at 300g and discard the supernatant. Further steps perform on ice and with ice-cold reagents. 3) Resuspend the cells in 5 ml of cold fixation solution (Miltenyi Biotec) and incubate for 30 min. on ice. 4) Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. 5) Resuspend the cells in 5 ml of ice-cold PBS with 1% BSA. 6) Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. 7) Resuspend the cells in 5 ml of ice-cold permeabilization solution (Miltenyi Biotec) and incubate 5 min. 8) Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. 9) Resuspend the cells in μl of ice-cold permeabilization solution and add 20 μl of FcR blocking solution and incubate for 5 min. (4°C, in the dark). 10) Perform intracellular staining of Helios for 30 min. (4°C, in the dark) with 10 μl of anti-Helios PE antibody (22F6) in 100 μl. 11) Add 2 ml of ice-cold permeabilization solution and incubate for 5 min. at 4°C in the dark. 12) Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. 13) Resuspend the cells in 3 ml of ice-cold PBS with 1% BSA. 14) Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. 15) Resuspend the cells in 150 μl of ice-cold PBS with 1% BSA and measure on a flow cytometry device with appropriate setting as soon as possible. Keep cold until measuring.
• Antibody Type: Primary Antibody
• Clone Number: 22F6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ANF1A2, IKZF2
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 22807

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-Helios (22F6) purified antibody (concentration in sample 8 μg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-Helios (22F6) purified antibody (concentration in sample 8 μg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-Helios (22F6) purified antibody (concentration in sample 8 μg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-Helios (22F6) purified antibody (concentration in sample 8 μg/ml, GAM APC).