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Item 1 of 4
Item 1 of 4
Anti-Helios Purified
Catalog Number: orb222726
| Catalog Number | orb222726 |
|---|---|
| Category | Antibodies |
| Description | Hamster monoclonal antibody to Helios |
| Clonality | Monoclonal |
| Clone Number | 22F6 |
| Tested applications | FC |
| Reactivity | Human, Mouse |
| Isotype | Armenian Hamster IgG |
| Immunogen | Peptide coresponding to the amino acids 51-107 of Helios |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | Helios |
| Entrez | 22807 |
| UniProt ID | Q9UKS7 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-ANF1A2 antibody, anti-IKZF2 antibody Read more... |
| Note | For research use only |
| Application notes | Flow cytometry: Recommended dilution: 1-4 μg/ml.,Recommended protocol: 1) Perform staining of cell surface markers (CD25, CD4 etc.) for 20 min. at room temperature in the dark; 100 μl of peripheral blood. 2) Add 3 ml of PBS with 1% BSA, centrifugate at 300g and discard the supernatant. Further steps perform on ice and with ice-cold reagents. 3) Resuspend the cells in 5 ml of cold fixation solution (Miltenyi Biotec) and incubate for 30 min. on ice. 4) Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. 5) Resuspend the cells in 5 ml of ice-cold PBS with 1% BSA. 6) Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. 7) Resuspend the cells in 5 ml of ice-cold permeabilization solution (Miltenyi Biotec) and incubate 5 min. 8) Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. 9) Resuspend the cells in μl of ice-cold permeabilization solution and add 20 μl of FcR blocking solution and incubate for 5 min. (4°C, in the dark). 10) Perform intracellular staining of Helios for 30 min. (4°C, in the dark) with 10 μl of anti-Helios PE antibody (22F6) in 100 μl. 11) Add 2 ml of ice-cold permeabilization solution and incubate for 5 min. at 4°C in the dark. 12) Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. 13) Resuspend the cells in 3 ml of ice-cold PBS with 1% BSA. 14) Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. 15) Resuspend the cells in 150 μl of ice-cold PBS with 1% BSA and measure on a flow cytometry device with appropriate setting as soon as possible. Keep cold until measuring. |
| Expiration Date | 12 months from date of receipt. |
Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-Helios (22F6) purified antibody (concentration in sample 8 μg/ml, GAM APC).
Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-Helios (22F6) purified antibody (concentration in sample 8 μg/ml, GAM APC).
Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-Helios (22F6) purified antibody (concentration in sample 8 μg/ml, GAM APC).
Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-Helios (22F6) purified antibody (concentration in sample 8 μg/ml, GAM APC).
