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Catalog Number | orb179854 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to AGPS. |
Target | AGPS |
Clonality | Monoclonal |
Isotype | Mouse IgG2a |
Conjugation | Unconjugated |
Reactivity | Human |
Concentration | 1 mg/ml |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Purity | Purified by protein-A affinity chromatography. |
Immunogen | recombinant human AGPS (amino acids 158-384) |
UniProt ID | O00116 |
Tested applications | FC, WB |
Application notes | Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining. |
Antibody Type | Primary Antibody |
Clone Number | AGPS-03 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Anti-ADAS antibody, anti-ADPS antibody, anti-RCDP3 Read more... |
Note | For research use only |
Entrez | 8540 |
Separation of K562 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood spiked with K562 cells stained using anti-AGPS (AGPS-03) purified antibody (concentration in sample 5 µg/ml, GAM APC).
Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with K562 cells stained using anti-AGPS (AGPS-03) purified antibody (concentration in sample 5 µg/ml, GAM APC).
Western blotting analysis of human AGPS using mouse monoclonal antibody AGPS-03 on lysates of HEP-G2 and Molt-4 cells, and MCF-7 cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-AGPS monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. AGPS was detected at approximately 70 kDa.