AGPS Antibody

SKU: orb179854

Description

Mouse monoclonal antibody to AGPS.

Images & Validation

• Tested Applications: FC, WB
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.Western blotting: Recommended dilution: 1-2 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: AGPS-03
• Immunogen: recombinant human AGPS (amino acids 158-384)
• Target: AGPS
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

ADAS, ADPS, RCDP3, ADAP-S, ADHAPS, ALDHPSY

Separation of K562 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood spiked with K562 cells stained using anti-AGPS (AGPS-03) purified antibody (concentration in sample 5 µg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with K562 cells stained using anti-AGPS (AGPS-03) purified antibody (concentration in sample 5 µg/ml, GAM APC).

Western blotting analysis of human AGPS using mouse monoclonal antibody AGPS-03 on lysates of HEP-G2 and Molt-4 cells, and MCF-7 cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-AGPS monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. AGPS was detected at approximately 70 kDa.

Quick Database Links

Gene Symbol

AGPS

UniProt

O00116

NCBI Gene ID

8540