AGPS Antibody (APC)

• Catalog Number: orb1821022
• Category: Antibodies
• Description: AGPS (alkylglycerone phosphate synthase), is an enzyme that catalyzes the second step of ether lipid biosynthesis in which acyl-dihydroxyacetone phosphate (acyl-DHAP) is converted to alkyl-DHAP by addition of a long chain alcohol and removal of a long-chain acid anion. The protein is localized to the inner side of the peroxisomal membrane and requires FAD as a cofactor. Mutations in AGPS gene have been associated with type 3 of rhizomelic chondrodysplasia punctata (RCDP3), and Zellweger syndrome. Higher expression of AGPS was observed in BCR/ABL positive leukemias and it was also described to be associated with higher risk of relapse.
• Target: AGPS
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Conjugation: APC
• Reactivity: Human
• Concentration: 0.1 mg/ml
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: recombinant human AGPS (amino acids 158-384)
• UniProt ID: O00116
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
• Clone Number: AGPS-03
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: ADAS, ADPS, RCDP3, ADAP-S, ADHAPS, ALDHPSY
• Note: For research use only
• Entrez: 8540
• Expiration Date: 12 months from date of receipt.

Separation of A431 cells stained using anti-AGPS (MHD4-46) APC antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from A431 cells stained using mouse IgG2a isotype control (MOPC-173) APC antibody (concentration in sample 5 μg/ml, same as AGPS APC antibody concentration, black-dashed) in flow cytometry analysis (intracellular staining).