Amyloid Precursor Protein Mouse Polyclonal Antibody

• Catalog Number: orb10118
• Category: Antibodies
• Description: Amyloid Precursor Protein Mouse Polyclonal Antibody
• Species/Host: Mouse
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Porcine
• Reactivity: Human, Rabbit, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from the middle of human Soluble APP-beta/alpha (381-480/770aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 72-83 kDa
• Target: APP
• UniProt ID: P05067
• RRID: AB_10767722
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Secretory Amyloid Precursor Protein; Soluble APP-a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Amyloid Precursor Protein) Monoclonal Antibody, Unconjugated (orb10118) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Human U87MG Cell Lysates, Lane 2: Human SY5Y cell lysates, Lane 3: Human U251 Cell Lysates, Primary: Anti-beta Amyloid 1-16 (orb10118) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 4.3/83 kDa, Observed band size: 130 kDa.

Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Amyloid Precursor Protein Polyclonal Antibody, Unconjugated (orb10118) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.